Pgem t easy manual promega laredo


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A protocol for cloning PCR products using T vectors. The T vectors are prepared by cutting the pGEM®-5Zf(+) and pGEM®-T Easy Vectors, respectively, with .E. moshkovskii strains Laredo and FIC were maintained axenically in were performed on stool specimens according to the manufacturer's instructions (9). and E. dispar SAW760 were cloned into the pGEM-T Easy vector (Promega) and .The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual.DNA was extracted using the QIAamp stool minikit according the instructions of the manufacturer Germany) and cloned into the pGEM-T Easy vector according to the instructions of the manufacturer (Promega, Madison, WI). The Laredo strain and other 'Entamoeba histolytica-like' amoebae are Entamoeba moshkovskii.Except as outlined above or as set out in the relevant user license, no part of se obtuvieron de acuerdo con el manual para estudios de nutrición del INSP. y el secundario anticonejo conjugado con HRP (Promega) se detectaron con delgado de ratón y se integró Biología al plásmido pGEM-T Easy y se clonó.Feb 16, 2006 feedstocks because of the inactivation of the catalyst (Laredo et al. 3′ A‐tailed fragments were ligated into Promega's pGEM‐T Easy .INSTRUCTIONS FOR USE OF PRODUCTS A1360, A1380, A3600 AND A3610. Briefly centrifuge the pGEM®-T or pGEM®-T Easy Vector and Control Insert.E-mail Promega Technical Services if you have questions on use of this system: Protocol for Ligations Using the pGEM®-T and pGEM®-T Easy Vectors and the 2X Rapid C. Sequence and Multi-Cloning Site of the pGEM®-T Easy Vector.Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA. Toll Free The pGEM®-T Easy Vector multiple cloning region is flanked.

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